How Long to Read Hidden Treasures in Contemporary RNA Sequencing

How Long Does it Take to Read Hidden Treasures in Contemporary RNA Sequencing?

It takes the average reader 1 hour and 34 minutes to read Hidden Treasures in Contemporary RNA Sequencing by Serghei Mangul

Assuming a reading speed of 250 words per minute. Learn more

Description

Advances in RNA-sequencing (RNA-seq) technologies have provided an unprecedented opportunity to explore the gene expression landscape across individuals, tissues, and environments by efficiently profiling the RNA sequences present in the samples. When a reference genome sequence or a transcriptome of the sample is available, mapping-based RNA-seq analysis protocols align the RNA-seq reads to the reference sequences, identify novel transcripts, and quantify the abundance of expressed transcripts.The reads that fail to map to the human reference, known as unmapped reads, are a large and often overlooked output of standard RNA-seq analyses. Even in carefully executed experiments, the unmapped reads can comprise a considerable fraction of the complete set of reads produced, and can arise due to technical sequencing produced by low-quality and error-prone copies of the nascent RNA sequence being sampled. Reads can also remain unmapped due to unknown transcripts, recombined B and T cell receptor sequences, A-to-G mismatches from A-to-I RNA editing, trans-splicing, gene fusion, circular RNAs, and the presence of non-host RNA sequences (e.g. bacterial, fungal, and viral organisms). Unmapped reads represent a rich resource for the study of B and T cell receptor repertoires and the human microbiome system—without incurring the expense of additional targeted sequencing.This book introduces and describes the Read Origin Protocol (ROP), a tool that identifies the origin of both mapped and unmapped reads. The protocol first identifies human reads using a standard high-throughput algorithm to map them onto a reference genome and transcriptome. After alignment, reads are grouped into genomic (e.g. CDS, UTRs, introns) and repetitive (e.g. SINEs, LINEs, LTRs) categories. The rest of the ROP protocol characterizes the remaining unmapped reads, which failed to map to the human reference sequences.

How long is Hidden Treasures in Contemporary RNA Sequencing?

Hidden Treasures in Contemporary RNA Sequencing by Serghei Mangul is 93 pages long, and a total of 23,529 words.

This makes it 31% the length of the average book. It also has 29% more words than the average book.

How Long Does it Take to Read Hidden Treasures in Contemporary RNA Sequencing Aloud?

The average oral reading speed is 183 words per minute. This means it takes 2 hours and 8 minutes to read Hidden Treasures in Contemporary RNA Sequencing aloud.

What Reading Level is Hidden Treasures in Contemporary RNA Sequencing?

Hidden Treasures in Contemporary RNA Sequencing is suitable for students ages 10 and up.

Note that there may be other factors that effect this rating besides length that are not factored in on this page. This may include things like complex language or sensitive topics not suitable for students of certain ages.

When deciding what to show young students always use your best judgement and consult a professional.

Where Can I Buy Hidden Treasures in Contemporary RNA Sequencing?

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