How Long to Read Optimization of an Affinity Purification - Mass Spectrometry Pipeline and Characterization of the Rub1p and Smt3p Interactomes

How Long Does it Take to Read Optimization of an Affinity Purification - Mass Spectrometry Pipeline and Characterization of the Rub1p and Smt3p Interactomes?

It takes the average reader 4 hours to read Optimization of an Affinity Purification - Mass Spectrometry Pipeline and Characterization of the Rub1p and Smt3p Interactomes by Sarah Elizabeth Wheaton

Assuming a reading speed of 250 words per minute. Learn more

Description

The ubiquitin-like proteins (Ubls) are small polypeptides that function as post-translational modifications. Modification of a protein with a Ubl can alter its localization, activity and/or half-life. SUMO and Rub1p/Nedd8 are two Ubls that play important roles in a number of critical cellular processes, yet their specific cellular functions remain poorly understood. To better understand these important Ubls, we developed a robust affinity purification-mass spectrometry (AP-MS) technique to generate protein-protein interaction maps for the Ubl systems. Each bait was systematically expressed as a C-terminal HA-tagged fusion protein in S. cerevisiae. A standardized method in which affinity purification via the HA epitope, followed by mild washing and mass spectrometric analysis, was performed and the data generated were used to build interaction maps. Affinity purification of the Rub1p E3 ligase Dcn1p identified a novel interaction with the AAA ATPase Cdc48p. This interaction was further studied to determine its biological significance.

How long is Optimization of an Affinity Purification - Mass Spectrometry Pipeline and Characterization of the Rub1p and Smt3p Interactomes?

Optimization of an Affinity Purification - Mass Spectrometry Pipeline and Characterization of the Rub1p and Smt3p Interactomes by Sarah Elizabeth Wheaton is 240 pages long, and a total of 60,000 words.

This makes it 81% the length of the average book. It also has 73% more words than the average book.

How Long Does it Take to Read Optimization of an Affinity Purification - Mass Spectrometry Pipeline and Characterization of the Rub1p and Smt3p Interactomes Aloud?

The average oral reading speed is 183 words per minute. This means it takes 5 hours and 27 minutes to read Optimization of an Affinity Purification - Mass Spectrometry Pipeline and Characterization of the Rub1p and Smt3p Interactomes aloud.

What Reading Level is Optimization of an Affinity Purification - Mass Spectrometry Pipeline and Characterization of the Rub1p and Smt3p Interactomes?

Optimization of an Affinity Purification - Mass Spectrometry Pipeline and Characterization of the Rub1p and Smt3p Interactomes is suitable for students ages 12 and up.

Note that there may be other factors that effect this rating besides length that are not factored in on this page. This may include things like complex language or sensitive topics not suitable for students of certain ages.

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