How Long to Read Regulation of Actin Dynamics by SelR

How Long Does it Take to Read Regulation of Actin Dynamics by SelR?

It takes the average reader and 54 minutes to read Regulation of Actin Dynamics by SelR by Aaron E. Miller (Graduate student)

Assuming a reading speed of 250 words per minute. Learn more

Description

Abstract: Regulation of actin polymerization and disassembly by the Mical/SelR enzyme pair has been shown to play an important role in abscission, the terminal step of cytokinesis, helping to prevent multiploidy. Oxidation of filamentous actin by the Mical family of monooxygenases promotes its disassembly, and reduction of actin by methionine sulfoxide reductase B (MsrB/SelR) promotes actin repolymerization. However, this process is poorly understood mechanistically. For instance, it is not known which forms of actin preferentially interact with SelR. Likewise, little is known about the enzymatic reaction of Mical-oxidized (Mox-) actin reduction catalyzed by SelR. In this study, SelR-C124S, a catalytically dead mutant, was created and validated as a tool for studying SelR binding to Mox-actin. The expression and purification protocols for recombinant SelR proteins were developed and optimized. This study provided the first insights on SelR binding to different forms of actin, assessed in analytical ultracentrifugation, native gel electrophoresis, and high-speed pelleting assays. The effectiveness of two reducing agents in supporting the catalytic activity of SelR in vitro were evaluated. Both reducing reagents were shown to be effective at lower concentrations than originally expected; tris(2-carboxyethyl)phosphine (TCEP) was shown to be a more efficient reductant compared to Dithiothreitol (DTT). These findings provided new insights on SelR interaction with actin.

How long is Regulation of Actin Dynamics by SelR?

Regulation of Actin Dynamics by SelR by Aaron E. Miller (Graduate student) is 54 pages long, and a total of 13,716 words.

This makes it 18% the length of the average book. It also has 17% more words than the average book.

How Long Does it Take to Read Regulation of Actin Dynamics by SelR Aloud?

The average oral reading speed is 183 words per minute. This means it takes 1 hour and 14 minutes to read Regulation of Actin Dynamics by SelR aloud.

What Reading Level is Regulation of Actin Dynamics by SelR?

Regulation of Actin Dynamics by SelR is suitable for students ages 8 and up.

Note that there may be other factors that effect this rating besides length that are not factored in on this page. This may include things like complex language or sensitive topics not suitable for students of certain ages.

When deciding what to show young students always use your best judgement and consult a professional.

Where Can I Buy Regulation of Actin Dynamics by SelR?

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