How Long to Read Characterization of the Putative Beta-1,6-poly-N-Acetylglucosamine De-N-acetylase Enzyme IcaB from Staphylococcus Epidermidis

How Long Does it Take to Read Characterization of the Putative Beta-1,6-poly-N-Acetylglucosamine De-N-acetylase Enzyme IcaB from Staphylococcus Epidermidis?

It takes the average reader 1 hour and 20 minutes to read Characterization of the Putative Beta-1,6-poly-N-Acetylglucosamine De-N-acetylase Enzyme IcaB from Staphylococcus Epidermidis by Heather Irene Griffiths

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Description

IcaB is a putative de-N-acetylase enzyme that is expressed in biofilm forming strains of Staphylococcus epidermidis. Previous studies showed that IcaB mutant strains of S. epidermidis were unable to deacetylate the exopolysaccharide beta-1,6-poly-N-Acetylglucosamine (PIA), a major component of the matrix that allows the cells to aggregate during biofilm formation. Although these studies proved that IcaB was involved in PIA deacetylation, its specific role as a deacetylase enzyme was not confirmed and required additional studies.We used in vitro-based experiments to characterize the function of IcaB. These studies require pure IcaB. Recombinant IcaB containing was expressed at 15 °C and purified at 4 °C to increase the yield of soluble protein. Multiple buffer, and salt conditions, as well as specific additives were tested to optimize solubility of IcaB. Although progress was made to find conditions that favored IcaB solubility, IcaB did not show any deacetylase activity in vitro.

How long is Characterization of the Putative Beta-1,6-poly-N-Acetylglucosamine De-N-acetylase Enzyme IcaB from Staphylococcus Epidermidis?

Characterization of the Putative Beta-1,6-poly-N-Acetylglucosamine De-N-acetylase Enzyme IcaB from Staphylococcus Epidermidis by Heather Irene Griffiths is 78 pages long, and a total of 20,124 words.

This makes it 26% the length of the average book. It also has 25% more words than the average book.

How Long Does it Take to Read Characterization of the Putative Beta-1,6-poly-N-Acetylglucosamine De-N-acetylase Enzyme IcaB from Staphylococcus Epidermidis Aloud?

The average oral reading speed is 183 words per minute. This means it takes 1 hour and 49 minutes to read Characterization of the Putative Beta-1,6-poly-N-Acetylglucosamine De-N-acetylase Enzyme IcaB from Staphylococcus Epidermidis aloud.

What Reading Level is Characterization of the Putative Beta-1,6-poly-N-Acetylglucosamine De-N-acetylase Enzyme IcaB from Staphylococcus Epidermidis?

Characterization of the Putative Beta-1,6-poly-N-Acetylglucosamine De-N-acetylase Enzyme IcaB from Staphylococcus Epidermidis is suitable for students ages 10 and up.

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