How Long to Read Identification and Characterization of a Novel Phosphoregulatory Site on Cyclin-dependent Kinase 5

How Long Does it Take to Read Identification and Characterization of a Novel Phosphoregulatory Site on Cyclin-dependent Kinase 5?

It takes the average reader 1 hour and 23 minutes to read Identification and Characterization of a Novel Phosphoregulatory Site on Cyclin-dependent Kinase 5 by Brett Lee Roach

Assuming a reading speed of 250 words per minute. Learn more

Description

Abstract: Reversible protein phosphorylation serves as a key mechanism to regulate virtually every cellular process by altering the activation status, subcellular location, stability and/or protein-protein interactions of the target protein. Protein kinases, the enzymes that catalyze the phosphorylation reaction, are often themselves subject to phosphoregulation. Cyclin-dependent kinase 5 (CDK5) is a proline-directed serine/threonine kinase essential for embryonic development whose over-activation has been implicated in several pathologies including neuro-degeneration, cancer cell metastasis and type II diabetes. Therefore, it is important to investigate molecular mechanism(s) that mediate regulation of CDK5 activity. Here, we identify and characterize a novel phosphoregulatory site on CDK5. Our mass spectrometry analysis identified seven putative phosphorylation sites on CDK5. Using phosphomimetic and non-phosphorylatable mutants, we determined that phosphorylation of S47, one of the identified sites, renders the kinase catalytically inactive. The inactivation of the kinase due to the phosphomimetic change at S47 results from the inhibition of its interaction with its cognate activator, p35 as determined by co-immunoprecipitation assays. Finally, we connect the effect of this regulatory event to a cellular phenotype by showing that the S47D CDK5 mutant inhibits cell migration. Together, these results have uncovered a potential physiological mechanism to regulate CDK5 activity. The evolutionary conservation of this residue in not only CDK5, but also in other CDK family members suggests that this phosphosite may represent a shared regulatory mechanism across the CDK family.

How long is Identification and Characterization of a Novel Phosphoregulatory Site on Cyclin-dependent Kinase 5?

Identification and Characterization of a Novel Phosphoregulatory Site on Cyclin-dependent Kinase 5 by Brett Lee Roach is 83 pages long, and a total of 20,999 words.

This makes it 28% the length of the average book. It also has 26% more words than the average book.

How Long Does it Take to Read Identification and Characterization of a Novel Phosphoregulatory Site on Cyclin-dependent Kinase 5 Aloud?

The average oral reading speed is 183 words per minute. This means it takes 1 hour and 54 minutes to read Identification and Characterization of a Novel Phosphoregulatory Site on Cyclin-dependent Kinase 5 aloud.

What Reading Level is Identification and Characterization of a Novel Phosphoregulatory Site on Cyclin-dependent Kinase 5?

Identification and Characterization of a Novel Phosphoregulatory Site on Cyclin-dependent Kinase 5 is suitable for students ages 10 and up.

Note that there may be other factors that effect this rating besides length that are not factored in on this page. This may include things like complex language or sensitive topics not suitable for students of certain ages.

When deciding what to show young students always use your best judgement and consult a professional.

Where Can I Buy Identification and Characterization of a Novel Phosphoregulatory Site on Cyclin-dependent Kinase 5?

Identification and Characterization of a Novel Phosphoregulatory Site on Cyclin-dependent Kinase 5 by Brett Lee Roach is sold by several retailers and bookshops. However, Read Time works with Amazon to provide an easier way to purchase books.

To buy Identification and Characterization of a Novel Phosphoregulatory Site on Cyclin-dependent Kinase 5 by Brett Lee Roach on Amazon click the button below.