How Long to Read Invertebrate Tissue Culture Methods

How Long Does it Take to Read Invertebrate Tissue Culture Methods?

It takes the average reader 7 hours and 36 minutes to read Invertebrate Tissue Culture Methods by Jun Mitsuhashi

Assuming a reading speed of 250 words per minute. Learn more

Description

I started insect cell culture work in 1962, when T. D. C. Grace reported the first establishment of invertebrate continuous cell lines. He obtained grow ing cells from pupal ovaries of the emperor gum moth, Antheraea euca lypti. At that time, I was trying to obtain growing cells from leafhoppers. Grace's method could not be applied directly to my culture because of the differences in species, the size of the insects, and the tissue to be cul tured. The vertebrate tissue culture methods gave me some ideas for pre paring cultures from leafhoppers, but those could not be used directly either. There were no textbooks and no manuals for invertebrate tissue culture, so I had to develop a method by myself. First, I considered what type and what size of vessels are suitable for insect tissue culture. Also, I had to look for suitable materials to construct the culture vessels. Sec ond, I had to examine various culture media, especially growth-promot ing substances, such as sera. Then I had to improve culture media by trial and error. The procedure to set up a primary culture was also a problem. How could I sterilize materials? How could I remove tissues from a tiny insect? How many tissues should I pool in order to set up one culture? I had to find out the answers. Naturally, it took a lot of time.

How long is Invertebrate Tissue Culture Methods?

Invertebrate Tissue Culture Methods by Jun Mitsuhashi is 446 pages long, and a total of 114,176 words.

This makes it 151% the length of the average book. It also has 140% more words than the average book.

How Long Does it Take to Read Invertebrate Tissue Culture Methods Aloud?

The average oral reading speed is 183 words per minute. This means it takes 10 hours and 23 minutes to read Invertebrate Tissue Culture Methods aloud.

What Reading Level is Invertebrate Tissue Culture Methods?

Invertebrate Tissue Culture Methods is suitable for students ages 12 and up.

Note that there may be other factors that effect this rating besides length that are not factored in on this page. This may include things like complex language or sensitive topics not suitable for students of certain ages.

When deciding what to show young students always use your best judgement and consult a professional.

Where Can I Buy Invertebrate Tissue Culture Methods?

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