How Long to Read Kinetic Analysis of a Series of Phosphotriesterase Mutants with Respect to Sarin and Soman Catalysis

By Steven P. Harvey

How Long Does it Take to Read Kinetic Analysis of a Series of Phosphotriesterase Mutants with Respect to Sarin and Soman Catalysis?

It takes the average reader 1 hour and 5 minutes to read Kinetic Analysis of a Series of Phosphotriesterase Mutants with Respect to Sarin and Soman Catalysis by Steven P. Harvey

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Description

A series of site-directed mutants of the organophosphorus-hydrolyzing enzyme phosphotriesterase (PTE) were analyzed with respect to their kinetics on the G-agent substrates sarin (GB) and soman (GD). Values of Km, Vmax, and Kcat/K m were compared with the wild-type values. Several mutants were found with decreased Km values on both substrates, although all had at least somewhat decreased Vmax values as well. The K cat/Km calculations showed two mutants (G6OA and H254G/H257R) with GB catalytic efficiencies nearly as high as wild- type. With respect to GD catalytic efficiency, both these mutants were in the same Kcat/Km range or higher than wild-type. In addition to their potential practical utility for decontamination, these results also provide part of the roadmap for future mutagenesis studies with the PTE enzyme.

How long is Kinetic Analysis of a Series of Phosphotriesterase Mutants with Respect to Sarin and Soman Catalysis?

Kinetic Analysis of a Series of Phosphotriesterase Mutants with Respect to Sarin and Soman Catalysis by Steven P. Harvey is 64 pages long, and a total of 16,256 words.

This makes it 22% the length of the average book. It also has 20% more words than the average book.

How Long Does it Take to Read Kinetic Analysis of a Series of Phosphotriesterase Mutants with Respect to Sarin and Soman Catalysis Aloud?

The average oral reading speed is 183 words per minute. This means it takes 1 hour and 28 minutes to read Kinetic Analysis of a Series of Phosphotriesterase Mutants with Respect to Sarin and Soman Catalysis aloud.

What Reading Level is Kinetic Analysis of a Series of Phosphotriesterase Mutants with Respect to Sarin and Soman Catalysis?

Kinetic Analysis of a Series of Phosphotriesterase Mutants with Respect to Sarin and Soman Catalysis is suitable for students ages 8 and up.

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