How Long to Read Optical Resonant Nanoprobes for the Measurements of Biomolecular Interactions

How Long Does it Take to Read Optical Resonant Nanoprobes for the Measurements of Biomolecular Interactions?

It takes the average reader 1 hour and 52 minutes to read Optical Resonant Nanoprobes for the Measurements of Biomolecular Interactions by Sanja Zlatanovic

Assuming a reading speed of 250 words per minute. Learn more

Description

Quantifying the interactions between biomolecules combined with discovering their structure provides better understanding of the underlying molecular mechanisms and opens the capability to accurately predict and design micromolecular structures and interactions. The ability of this approach to advancing the diagnostics and the treatment of disease is immense. In this thesis, it was demonstrated that two resonant optical nanoprobes, namely photonic crystal microcavity sensor and plasmon-coupled nanoparticle probe can be applied toward investigating biomolecular interactions. Both techniques are based on the measurements of optical resonances. Measurement of protein binding kinetics using photonic crystal microcavity sensor was demonstrated for the first time. Real-time monitoring of the resonant wavelength provides information on the strength of protein binding and concentration. The sensor performance was demonstrated with biotinylated-BSA and anti-biotin. Mass transport of molecules to the sensing surface was analyzed to explain the relatively long transition time needed to reach the binding equilibrium in time resolved experiments. Binding of small molecular species such as aromatic rings was detected. The detection limit, in terms of the mass of molecules bound to the surface, was shown to be less than 4.5fg. The small modal volume and photonic confinement inside the microcavity enable detection of attoliter samples. The calculations show that the response of the sensor to binding of a single molecule is 0.72pm. By implementing temperature control and signal processing techniques, signal-to-noise ratio can be improved to allow for single molecule detection. Plasmon coupled-nanoparticle probes were used to measure the binding strength between two DNA strands, with the idea to discern single nucleotide mismatches in the sequence. The probe consists of two streptavidin-coated gold nanoparticles interconnected with two biotinylated-DNA strands under test. An external Coulombic force was applied by lowering the ionic concentration of the solution, causing the binding strength between complementary DNA strands to be weakened. This was converted to a distance change between plasmon-coupled gold nanoparticles, causing a shift in their resonant wavelength position. This is a new approach to the measurement of the binding strength within molecular complexes.

How long is Optical Resonant Nanoprobes for the Measurements of Biomolecular Interactions?

Optical Resonant Nanoprobes for the Measurements of Biomolecular Interactions by Sanja Zlatanovic is 112 pages long, and a total of 28,224 words.

This makes it 38% the length of the average book. It also has 34% more words than the average book.

How Long Does it Take to Read Optical Resonant Nanoprobes for the Measurements of Biomolecular Interactions Aloud?

The average oral reading speed is 183 words per minute. This means it takes 2 hours and 34 minutes to read Optical Resonant Nanoprobes for the Measurements of Biomolecular Interactions aloud.

What Reading Level is Optical Resonant Nanoprobes for the Measurements of Biomolecular Interactions?

Optical Resonant Nanoprobes for the Measurements of Biomolecular Interactions is suitable for students ages 10 and up.

Note that there may be other factors that effect this rating besides length that are not factored in on this page. This may include things like complex language or sensitive topics not suitable for students of certain ages.

When deciding what to show young students always use your best judgement and consult a professional.

Where Can I Buy Optical Resonant Nanoprobes for the Measurements of Biomolecular Interactions?

Optical Resonant Nanoprobes for the Measurements of Biomolecular Interactions by Sanja Zlatanovic is sold by several retailers and bookshops. However, Read Time works with Amazon to provide an easier way to purchase books.

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