How Long to Read Protein Identification Via Assembly of Tandem Mass Spectra

How Long Does it Take to Read Protein Identification Via Assembly of Tandem Mass Spectra?

It takes the average reader 1 hour and 51 minutes to read Protein Identification Via Assembly of Tandem Mass Spectra by Adrian Lewis Guthals

Assuming a reading speed of 250 words per minute. Learn more

Description

High-throughput proteomics is made possible by a combination of modern mass spectrometry instruments capable of generating many millions of tandem mass (MS2 or MS/MS) spectra on a daily basis and the increasingly sophisticated associated software for their automated identification. Despite the growing accumulation of collections of identified spectra and the regular generation of MS2 data from related peptides, the mainstream approach for peptide identification is still the nearly two decades old approach of matching one MS2 spectrum at a time against a database of protein sequences. These traditional approaches fail for the identification of spectra from unknown proteins such as antibodies or proteins from organisms with un-sequenced genomes. Furthermore, attempts to identify MS/MS spectra against large databases (e.g., the human microbiome or 6-frame translation of the human genome) face a search space that is 10-100 times larger than the human proteome, where it becomes increasingly challenging to separate between true and false peptide matches. First, we describe techniques to utilize networks of spectra from related peptides to rigorously compute the joint spectral probability of multiple spectra being matched to peptides with overlapping sequences, thus improving peptide identification by 30-62% against large search spaces. We then introduce methods that dramatically improve de novo sequencing of unknown proteins using novel spectral network assembly algorithms and incorporating alternative MS/MS acquisition protocols. Finally, we describe an interesting end-goal biological problem for which the described advances in de novo sequencing can usher in a new era of therapeutic drug discovery.

How long is Protein Identification Via Assembly of Tandem Mass Spectra?

Protein Identification Via Assembly of Tandem Mass Spectra by Adrian Lewis Guthals is 108 pages long, and a total of 27,864 words.

This makes it 36% the length of the average book. It also has 34% more words than the average book.

How Long Does it Take to Read Protein Identification Via Assembly of Tandem Mass Spectra Aloud?

The average oral reading speed is 183 words per minute. This means it takes 2 hours and 32 minutes to read Protein Identification Via Assembly of Tandem Mass Spectra aloud.

What Reading Level is Protein Identification Via Assembly of Tandem Mass Spectra?

Protein Identification Via Assembly of Tandem Mass Spectra is suitable for students ages 10 and up.

Note that there may be other factors that effect this rating besides length that are not factored in on this page. This may include things like complex language or sensitive topics not suitable for students of certain ages.

When deciding what to show young students always use your best judgement and consult a professional.

Where Can I Buy Protein Identification Via Assembly of Tandem Mass Spectra?

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