How Long to Read The Interaction of Small Regulatory RNA with Model Globular Proteins

How Long Does it Take to Read The Interaction of Small Regulatory RNA with Model Globular Proteins?

It takes the average reader 1 hour and 30 minutes to read The Interaction of Small Regulatory RNA with Model Globular Proteins by Jeremiah Joseph Babcock

Assuming a reading speed of 250 words per minute. Learn more

Description

The interaction of proteins with a variety of biomolecular targets is central to the entire cellular machinery and, in a way, the mechanistic essence of life. Proteins can cooperatively bind with other polypeptides or oligonucleotides or assemble into aggregates prompted by specific environmental conditions. In the case of protein-oligonucleotide (RNA) binding, much research has been focused on specific RNA-binding proteins to the exclusion of more fundamental questions: 1) can model globular proteins non-covalently bind to double-stranded microRNA (miRNA) regardless of sequence identity or protein size and function and 2) can these model proteins be leveraged as transporters to deliver these short oligonucleotides for genetic therapy? In this dissertation, three works are presented, with an emphasis on biomolecular interactions, as investigated by fluorescence spectroscopy. The first study demonstrates the aggregation extent of bovine serum albumin over time, incubated in highly acidic (pH 2) or basic (pH 9) solvents at room temperature. The role of protein structural and charge density changes are discussed, with an emphasis on intrinsic fluorescence spectroscopic analysis. The second study investigates the appropriateness of the segmental mobility and rigid rotor fitting models applied to time-resolved fluorescence decay analysis. After a MD simulation of the fluorophore Atto 390 attached via the N-terminus to hen egg white lysozyme, intramolecular residual effects on the ligand's mobility are presented, with subsequent emphasis on the comparison of the experimental anisotropy decay with the computed theoretical decay. The last study examines the binding affinity of human serum albumin and hen egg white lysozyme to mimic double-stranded microRNA, miR106a, in physiological in vitro conditions before investigating the roles of salt and concentration in binding. Finally, a new way to induce miRNA binding to HSA via the F-N transition is introduced, making targeted miRNA delivery possible using a native blood transport protein.

How long is The Interaction of Small Regulatory RNA with Model Globular Proteins?

The Interaction of Small Regulatory RNA with Model Globular Proteins by Jeremiah Joseph Babcock is 90 pages long, and a total of 22,500 words.

This makes it 30% the length of the average book. It also has 27% more words than the average book.

How Long Does it Take to Read The Interaction of Small Regulatory RNA with Model Globular Proteins Aloud?

The average oral reading speed is 183 words per minute. This means it takes 2 hours and 2 minutes to read The Interaction of Small Regulatory RNA with Model Globular Proteins aloud.

What Reading Level is The Interaction of Small Regulatory RNA with Model Globular Proteins?

The Interaction of Small Regulatory RNA with Model Globular Proteins is suitable for students ages 10 and up.

Note that there may be other factors that effect this rating besides length that are not factored in on this page. This may include things like complex language or sensitive topics not suitable for students of certain ages.

When deciding what to show young students always use your best judgement and consult a professional.

Where Can I Buy The Interaction of Small Regulatory RNA with Model Globular Proteins?

The Interaction of Small Regulatory RNA with Model Globular Proteins by Jeremiah Joseph Babcock is sold by several retailers and bookshops. However, Read Time works with Amazon to provide an easier way to purchase books.

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