How Long to Read The SUV3/PNPase Complex Interacts with Mitochondrial Poly(A) Polymerase to Modulate the Poly(A) Tail Lengths of Mitochondrial RNA Transcripts in an Energy Dependent Manner

By Dennis Ding Hwa Wang

How Long Does it Take to Read The SUV3/PNPase Complex Interacts with Mitochondrial Poly(A) Polymerase to Modulate the Poly(A) Tail Lengths of Mitochondrial RNA Transcripts in an Energy Dependent Manner?

It takes the average reader 2 hours and 4 minutes to read The SUV3/PNPase Complex Interacts with Mitochondrial Poly(A) Polymerase to Modulate the Poly(A) Tail Lengths of Mitochondrial RNA Transcripts in an Energy Dependent Manner by Dennis Ding Hwa Wang

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Description

Mammalian mitochondrial RNA degradation, processing, and surveillance have long been ignored areas in biological science due to the obscure cellular localization and the seemingly lack of disease association. Furthermore, it has always been assumed that the mechanisms for RNA degradation/processing in the mitochondria are similar to that of prokaryotes since the endosymbiotic theory states that the mitochondria originated from free-living bacteria. However, the unique features of mitochondrial RNAs, including the lack of 5' caps and the presence of 2 distinct populations of 3' adenosine tails, suggest the machinery and regulations for these important biological processes are different from the prokaryotic and eukaryotic systems. A few players involved in RNA degradation, processing, and surveillance have been recently identified, including SUV3, PNPase and mtPAP. Genetics defects of these proteins have been reported to be involved in human diseases, which substantiate their importance in cellular and mitochondrial functions and homeostasis. This dissertation will first provide background information on mitochondrial diseases and genetics, including mini-reviews on mtDNA replication, transcription, translation, and the components of the putative RNA degradosome. Chapter 2 will discuss two unsuccessful attempts to establish RNase activities in SUV3 and mtPAP. The subsequent chapter will focus on the functional association between SUV3 and PNPase. In doing so, we showed that purified SUV3 and PNPase form a 330 kDa heteropentamer that is capable of degrading structured RNA in the presence of ATP. In the final chapter, a potential link between RNA processing and ATP production will be addressed by first establishing an interaction between the SUV3/PNPase complex with mtPAP, followed by examining the significance of this interaction in regards to the regulation of mtRNA poly(A) tail lengths and steady state levels. Information about experimental materials and methods is included in the appendix section.

How long is The SUV3/PNPase Complex Interacts with Mitochondrial Poly(A) Polymerase to Modulate the Poly(A) Tail Lengths of Mitochondrial RNA Transcripts in an Energy Dependent Manner?

The SUV3/PNPase Complex Interacts with Mitochondrial Poly(A) Polymerase to Modulate the Poly(A) Tail Lengths of Mitochondrial RNA Transcripts in an Energy Dependent Manner by Dennis Ding Hwa Wang is 123 pages long, and a total of 31,119 words.

This makes it 42% the length of the average book. It also has 38% more words than the average book.

How Long Does it Take to Read The SUV3/PNPase Complex Interacts with Mitochondrial Poly(A) Polymerase to Modulate the Poly(A) Tail Lengths of Mitochondrial RNA Transcripts in an Energy Dependent Manner Aloud?

The average oral reading speed is 183 words per minute. This means it takes 2 hours and 50 minutes to read The SUV3/PNPase Complex Interacts with Mitochondrial Poly(A) Polymerase to Modulate the Poly(A) Tail Lengths of Mitochondrial RNA Transcripts in an Energy Dependent Manner aloud.

What Reading Level is The SUV3/PNPase Complex Interacts with Mitochondrial Poly(A) Polymerase to Modulate the Poly(A) Tail Lengths of Mitochondrial RNA Transcripts in an Energy Dependent Manner?

The SUV3/PNPase Complex Interacts with Mitochondrial Poly(A) Polymerase to Modulate the Poly(A) Tail Lengths of Mitochondrial RNA Transcripts in an Energy Dependent Manner is suitable for students ages 10 and up.

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